| A general purpose medium
that supports a wide variety of microorganism types and lacks inhibitory properties.
Enriched with an additive, usually animal blood, in order to cultivate highly fastidious
microbial species. |
| Procedure:
Inoculate the medium as soon as possible after the specimen arrives. To culture specimens
from swabs, inoculate the medium by rolling over a third of the agar surface and streaking
the remainder of the plate to obtain isolated colonies. Material not being cultured from
swabs may be streaked onto the medium with a sterilized inoculating loop. The streak plate
technique is used primarily to obtain isolated colonies from specimens containing mixed
flora. Incubate the plates in an inverted position (agar side up) at 35± 2ºC enriched
atmosphere for 18 to 48 hours. |
| Expected Results:
After 18 to 48 hours of incubation, the plates should show isolated colonies in streaked
areas and confluent growth in areas of heavy inoculation. |
